Ci-VSP Is A Depolarization-Activated PI(4,5)P2 And PI(3,4,5)P3 5' Phosphatase
نویسندگان
چکیده
منابع مشابه
Electro-chemical coupling in the voltage-dependent phosphatase Ci-VSP
In the voltage-sensing phosphatase Ci-VSP, a voltage-sensing domain (VSD) controls a lipid phosphatase domain (PD). The mechanism by which the domains are allosterically coupled is not well understood. Using an in vivo assay, we found that the interdomain linker that connects the VSD to the PD is essential for coupling the full-length protein. Biochemical assays showed that the linker is also n...
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The Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP) shares high homology with the phosphatidylinositol phosphatase enzyme known as PTEN (phosphatase and tensin homologue deleted on chromosome 10). We have taken advantage of the similarity between these proteins to inquire about the coupling between the voltage sensing and the phosphatase domains in Ci-VSP. Recently, it was sho...
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Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent ...
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The theory When reading a research report, the range of the CI provides assurance (or confi dence) regarding how precise the data are. CIs are calculated at a confi dence level, for example 95%. This level is predetermined by the researcher. Confi dence levels are usually calculated so that this percentage is 95% although others 90%, 99%, and 99.9% are sometimes applied. Researchers collect num...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.403